ABSTRACT
Introduction: Nosocomial infection is a major problem globally. Methicillin-resistant Staphylococcus aureus (MRSA) remains one of the most important causes of nosocomial infections worldwide. MRSA are the important agents causing nosocomial infections. The study was conducted in the Department of Microbiology to determine the prevalence of MRSA and antibiotic susceptibility pattern. Material and Methods: This was a retrospective study conducted from July 2016 to August 2017 in a tertiary care hospital in Northern Bihar India. All isolates were identified in patients and data provided by the Clinical and Laboratory Standards Institute (CLSI) guidelines and antibiotic susceptibility pattern considered by Kirby Bauer disc diffusion method. The information was definitely recorded and analyzed using Microsoft Excel 2007 edition. Results: 200 Staphylococcus strains isolate of were isolated from various clinical samples, Out of 200 S. aureus isolates, 73 (36.5%) were methicillin resistant S. aureus (MRSA) and 127 (63.5%) were methicillin-sensitive S. aureus (MSSA) in our labs. Although, the majority of the MRSA isolates were resulted from pus samples 30, however, the S. aureus isolates resulted from post-operative wound infection was mostly MRSA. Conclusion: In conclusion, the importance of Isolation of MRSA patients and carriers in the hospitals, regular surveillance of hospital associated infections including monitoring antibiotic sensitivity pattern and strict drug policy for antibiotics may be helpful for reducing the incidence of these infections
ABSTRACT
Introduction: Surgical site infections are most commonhospitals acquired infections and are an important cause ofmorbidity and mortality. The objective of our study is toconclude the causative bacteria and antimicrobial sensitivityof surgical site infections.Material and methods: A total of 275 various clinicalsamples received in Microbiology Laboratory, GovernmentMedical College, Bettiah (West Champaran) Bihar andAssociated Hospital. from March 2018 to April 2019. Atotal 101 Staphylococcus aureus isolated, were identifiedby standard biochemical methods. Antibiotic susceptibilitytesting was performed by Kirby Bauer Disc Diffusion method.Methicillin resistance was detected by using cefoxitin (30µg)disc diffusion method as per CLSI guidelines 2016.Result: Out of the 275 aerobic bacteria which were isolated,144 were gram positive cocci (52.37%) and 131 were gramnegative bacilli (47.63%). The most common pathogenfollowed by Staphylococcus aureus 101 (36.36%). Otherorganisms were Escherichia, Pseudomonas, Klebsiella,Citrobacter, Proteus, and Enterococcus. The Antimicrobialprofile of 101 Staphylococcus aureus isolates among MRSA,resistance those they were 100% sensitive to linezolidand vancomycin, with moderate sensitivity (71.14%) tocefuroxime, gentamicin and least sensitivity to (23.81%)doxycycline, (20.95%) ciprofloxacin.Conclusion: Isolation of MRSA patients and carriers in thehospitals, regular surveillance, and monitoring of antibioticsusceptibility pattern of the hospital and community of thatregion regularly and formulation of antibiotic policy may helpin reducing the treatment failures.